ABSTRACT
Objective: Based on the transcriptome sequencing results of Lycium barbarum under different concentrations of NaCl stress, the bHLH transcription factor family members of Lycium barbarum were identified by bioinformatics method. Methods: The bHLH family genes were screened by transcriptome sequencing of leaf and root samples of Lycium ruthenicum Murr. under NaCl stress. Results: The physicochemical properties, conserved domain, gene structure, cell location and phylogenetic development of these genes were analyzed by bioinformatics method. The results showed there were 89 bHLH family of Lycium ruthenicum. under NaCl stress. Their physicochemical properties were different, of which 71.90% proteins were weakly acidic and they were hydrophilic protein. The bHLH family of Lycium ruthenicum contained two conserved domains, which were located in the alkaline amino acid region of N and the helical ring and spiral region of C, respectively. The subcellular localization prediction of these bHLHs were mainly in the nucleus and extracellular. Phylogenetic analysis showed that 89 bHLH genes were divided into 20 subgroups, among which the 3 subgroup contained the most abundant bHLH members,including 11 bHLH proteins; the 7, 11, 13, 22 subgroups had only 1 member, respectively. In the other subgroups, the range was between 2 and 8. Conclusion: There are 89 members of bHLH transcription factor family in Lycium barbarum under NaCl stress, and most of the bHLH family proteins are weakly acidic, belonging to hydrophilic proteins, which can be divided into 20 subgroups.